Camellia sinensis and Phyllanthus amarus Ethanol Extracts Induced Apoptosis and Cell Cycle Arrest on Human Leukemic Cell Lines

Leukaemia is a heterogeneous hematologic malignancy characterized by unregulated proliferation of the early blood-forming cells which starts in bone marrow. The basic strategy leukaemia therapy involves induction leukemic apoptosis. Research on natural products have shown that some plant derivatives anticancer properties inducing apoptosis cells. Plants such as Camellia sinensis and Phyllanthus amarus are those had gained wide interest due to their anti-cancer effect. aim this study was investigate effects C. P. extracts human cell lines analysing cycle determining apoptotic state. were treated with ethanolic at concentrations 31.25 - 500 µg/mL for 24 h followed MTT assay determine IC50. IC50 U937 170±10.39 210±6.78 µg/mL, respectively. Flow cytometric analysis using Annexin V/propidium iodide (PI) staining also performed. extract 170 significantly increase U-937 (p<0.001), Jurkat (p<0.05) K-562 (p<0.01) when compared untreated Meanwhile, 210 induced both K562 but not caused arrest S phase (p<0.001) G0/G1 K652 control. Based findings, showed potential lines. In addition, has capability disrupt cycle.